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We may measure rotation curves for spiral galaxies and determine the masses of individual spiral
members of a cluster as we discussed earlier for our own galaxy. The rotation curves depend on
measuring Doppler effects for rotating galaxies.
For nearby galaxies one can resolve opposite sides of the galaxy and
measure the Doppler shift separately to estimate the rotation speed.
For galaxies further away the two sides cannot be resolved, but the
rotation leads to broadening of spectral lines. Thus, a careful study
of line broadening for distant spiral galaxies can be used to estimate rotational
speeds. Such studies indicate rotation curves very similar to those found for the
Milky Way, giving estimates for the mass that are factors of 10 or so larger than the
luminous matter.
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In principle, we can treat two galaxies in a cluster like we do binary stars and use
Kepler's laws to determine the mass of the "binary". In practice, the motion of galaxies
in clusters is too slow for us to follow the orbital motion, so this method cannot be
used directly. However, it can be used in an approximate way by measuring radial velocity
using Doppler methods and the present separation of the galaxies to estimate the orbit.
This is not very reliable for a single pair of galaxies, but we may expect it to be more
reliable when averaged over a whole set of pairs in a cluster, so it can give a reasonable
estimate of the total mass of the cluster.
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We can view the set of galaxies in the cluster as a gravitationally bound system. By
studying the average motion of the members of the cluster, we can ask how large a gravitational
field must be present to keep the system bound for long periods. The required gravitational
field can then be related to the total mass that generates it by Newton's gravitational law.
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All of these methods indicate large amounts non-luminous matter in clusters of galaxies. Further, they
indicate that the discrepancy between "unseen" and "seen" matter is as large as a factor of 10-100.